Background

The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3′ UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation.

Images

	Western blotting analysis using anti-lin-28 homolog B antibody (Cat#63753). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lin-28 homolog B antibody (Cat#63753, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-lin-28 homolog B antibody (Cat#63753). Lin-28 homolog B expression in wild-type (WT) and lin-28 homolog B (LIN28B) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-lin-28 homolog B antibody (Cat#63753, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Lin-28 homolog B expression in HAP-1 cells using anti-Lin-28 homolog B antibody (Cat#63753, 1:2,000). Green, isotype control; red, Lin-28 homolog B.
	Immunocytochemical staining of HAP1 cells with anti-Lin-28 homolog B antibody (Cat#63753, 1:1,000). Nuclei were stained blue with DAPI;Lin-28 homolog B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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