Background

This gene encodes a member of a large family of proteins which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor that is required for pituitary development and motor neuron specification. Mutations in this gene cause combined pituitary hormone deficiency 3. Alternative splicing results in multiple transcript variants encoding different isoforms.

Images

	Western blotting analysis using anti-LIM homeobox 3 antibody (Cat#61626). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LIM homeobox 3 antibody (Cat#61626, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-LIM homeobox 3 antibody (Cat#61626). LIM homeobox 3 expression in wild-type (WT) and LIM homeobox 3 (LHX3) knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LIM homeobox 3 antibody (Cat#61626, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of LIM homeobox 3 expression in C2C12 cells using anti-LIM homeobox 3 antibody (Cat#61626, 1:2,000). Green, isotype control; red, LIM homeobox 3.
	Immunocytochemical staining of C2C12 cells with anti-LIM homeobox 3 antibody (Cat#61626, 1:1,000). Nuclei were stained blue with DAPI; LIM homeobox 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

You may also be interested in: