Background

This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-LGI1 antibody (Cat#61141). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LGI1 antibody (Cat#61141, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-LGI1 antibody (Cat#61141). LGI1 expression in wild type (WT) and LGI1 shRNA knockdown (KD) HeLa cells with 20 μg of Total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LGI1 antibody (Cat#61141, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat# 201, 1:2,0000) respectively. Image was developed using Plus ECL Super Kit (Cat#MR1004).
	Flow cytometric analysis of LGI1 expression in HepG2 cells using LGI1 antibody (Cat#61141, 1:2,000). Green, isotype control; red, LGI1.
	Immunocytochemical staining of HepG2 cells with anti-LGI1 antibody (Cat#61141, 1:1,000). Nuclei were stained blue with DAPI; LGI1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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