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KD-Validated LGALS1 Rabbit pAb (20 μl)

$149.00

Cat. #: 64961

Description

Reactivity: Human, Mouse, Rat
Applications: WB
Host Species: Rabbit
Isotype: IgG
Clonality: Polyclonal antibody
Gene Name: galectin 1
Gene Symbol: LGALS1
Synonyms: GBP; GAL1
Gene ID: 3956
UniProt ID: P09382
Immunogen: A synthesized peptide derived from human Galectin 1
Dilution: WB 1:500-1:2,500
Purification Method: Affinity purified
Concentration: Lot dependent
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.


Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.


Images

LGALS1 antibody Western blotting analysis using anti-galectin 1 antibody (Cat#64961). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-galectin 1 antibody (Cat#64961, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
64961 WB KD 1 Western blotting analysis using anti-galectin 1 antibody (Cat#64961). Galectin 1 expression in wild-type (WT) and galectin 1 (LGALS1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-galectin 1 antibody (Cat#64961, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).


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