Description
| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | leucine aminopeptidase 3 |
| Gene Symbol: | LAP3 |
| Synonyms: | LAP; PEPS; LAPEP; HEL-S-106 |
| Gene ID: | 51056 |
| UniProt ID: | P28838 |
| Clone ID: | 24GB5965 |
| Immunogen: | A synthesized peptide derived from human LAP3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Predicted to enable peptidase activity. Predicted to be involved in proteolysis. Located in several cellular components, including extracellular exosome; focal adhesion; and mitochondrion.
Images
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Western blotting analysis using anti-leucine aminopeptidase 3 antibody (Cat#61144). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-leucine aminopeptidase 3 antibody (Cat#61144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-leucine aminopeptidase 3 antibody (Cat#61144). Leucine aminopeptidase 3 expression in wild type (WT) and leucine aminopeptidase 3 (LAP3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-leucine aminopeptidase 3 antibody (Cat#61144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of leucine aminopeptidase 3 expression in HAP-1 cells using anti-Leucine aminopeptidase 3 antibody (Cat#61144, 1:2,000). Green, isotype control; red, leucine aminopeptidase 3. |
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Immunocytochemical staining of HAP-1 cells with anti-leucine aminopeptidase 3 antibody (Cat#61144, 1:1,000). Nuclei were stained blue with DAPI; Leucine aminopeptidase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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