Description
| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | lysine demethylase 1B |
| Gene Symbol: | KDM1B |
| Synonyms: | AOF1; LSD2; C6orf193 |
| Gene ID: | 221656 |
| UniProt ID: | Q8NB78 |
| Clone ID: | 23GB6265 |
| Immunogen: | A synthesized peptide derived from human LSD2 / AOF1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).[supplied by OMIM, Oct 2009].
Images
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Western blotting analysis using anti-Lysine demethylase 1B antibody (Cat#62155). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lysine demethylase 1B antibody (Cat#62155, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-Lysine demethylase 1B antibody (Cat#62155). Lysine demethylase 1B expression in wild type (WT) and Lysine demethylase 1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Lysine demethylase 1B antibody (Cat#62155, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of Lysine demethylase 1B expression in HepG2 cells using Lysine demethylase 1B antibody (Cat#62155, 1:2,000). Green, isotype control; red, Lysine demethylase 1B. |
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Immunocytochemical staining of HepG2 cells with anti-Lysine demethylase 1B antibody (Cat#62155, 1:1,000). Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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