Background

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

Images

	Western blotting analysis using anti-IQGAP1 antibody (Cat#61278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IQGAP1 antibody (Cat#61278, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-IQGAP1 antibody (Cat#61278). IQGAP1 expression in wild-type (WT) and IQGAP1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IQGAP1 antibody (Cat#61278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of IQGAP1 expression in HepG2 cells using IQGAP1 antibody (Cat#61278, 1:2,000). Green, isotype control; red, IQGAP1.
	lmmunocytochemical staining of HepG2 cells with lQGAP1 antibody (Cat#61278, 1:1,000). Nuclei were stained blue with DAPl; IQGAP1 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

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