Background

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5′-monophosphate into xanthine-5′-monophosphate, which is then converted into guanosine-5′-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.

Images

	Western blotting analysis using anti-IMPDH2 antibody (Cat#61198). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IMPDH2 antibody (Cat#61198, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-IMPDH2 antibody (Cat#61198). IMPDH2 expression in wild type (WT) and IMPDH2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IMPDH2 antibody (Cat#61198, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of IMPDH2 expression in HT-1080 cells using IMPDH2 antibody (Cat#61198, 1:2,000). Green, isotype control; red, IMPDH2.
	Immunocytochemical staining of HT-1080 cells with anti-IMPDH2 antibody (Cat#61198, 1:1,000). Nuclei were stained blue with DAPI; IMPDH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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