Description
| Reactivity: | Human, Mouse |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | inhibitor of nuclear factor kappa B kinase subunit beta |
| Gene Symbol: | IKBKB |
| Synonyms: | IKK2; IKKB; IKK-2; IMD15; IMD15A; IMD15B; NFKBIKB; IKK-beta |
| Gene ID: | 3551 |
| UniProt ID: | O14920 |
| Clone ID: | 25GB2895 |
| Immunogen: | A synthesized peptide derived from human IKK beta |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene.
Images
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Western blotting analysis using anti-IKBKB antibody (Cat#61572). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IKBKB antibody (Cat#61572, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-IKBKB antibody (Cat#61572). IKBKB expression in wild type (WT) and IKBKB knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-IKBKB antibody (Cat#61572, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of IKBKB expression in HepG2 cells using anti-IKBKB antibody (Cat#61572, 1:2,000). Green, isotype control; red, IKBKB. |
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Immunocytochemical staining of HepG2 cells with anti-IKBK antibody (Cat#61572, 1:1,000). Nuclei were stained blue with DAPI; IKBK was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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