Background

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5′ UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.

Images

	Western blotting analysis using anti-IGF2BP3 antibody (Cat#62172). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IGF2BP3 antibody (Cat#62172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-IGF2BP3 antibody (Cat#62172). IGF2BP3 expression in wild type (WT) and IGF2BP3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IGF2BP3 antibody (Cat#62172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of IGF2BP3 expression in HepG2 cells using IGF2BP3 antibody (Cat#62172, 1:2,000). Green, isotype control; red, IGF2BP3.
	Immunocytochemical staining of HepG2 cells with anti-IGF2BP3 antibody (Cat#62172, 1:1,000). Nuclei were stained blue with DAPI; IGF2BP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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