Background

This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development. Intracellular or ectopic expression of this protein expands hematopoietic stem and progenitor cells in vivo and in vitro, making it a potential candidate for therapeutic stem cell expansion.

Images

	Western blotting analysis using anti-homeobox B4 antibody (Cat#61156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-homeobox B4 antibody (Cat#61156, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-homeobox B4 antibody (Cat#61156). Homeobox B4 expression in wild type (WT) and homeobox B4 (HOXB4) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-homeobox B4 antibody (Cat#61156, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of homeobox B4 expression in HAP-1 cells using anti-homeobox B4 antibody (Cat#61156, 1:2,000). Green, isotype control; red, homeobox B4.
	Immunocytochemical staining of HAP-1 cells with anti-Homeobox B4 antibody (Cat#61156, 1:1,000). Nuclei were stained blue with DAPI; Homeobox B4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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