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KD-Validated GRHPR Mouse mAb (20 μl)

$149.00

Cat. #: 63508

Description

Reactivity: Human
Applications: WB
Host Species: Mouse
Isotype: IgG2a
Clonality: Monoclonal antibody
Gene Name: glyoxylate and hydroxypyruvate reductase
Gene Symbol: GRHPR
Synonyms: PH2; GLXR; GLYD
Gene ID: 9380
UniProt ID: Q9UBQ7
Clone ID: 24GB6270
Immunogen: Recombinant protein of human GRHPR
Dilution: WB 1:2,500-1:5,000
Purification Method: Affinity purified
Concentration: Lot dependent
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.


Background

This gene encodes an enzyme with hydroxypyruvate reductase, glyoxylate reductase, and D-glycerate dehydrogenase enzymatic activities. The enzyme has widespread tissue expression and has a role in metabolism. Type II hyperoxaluria is caused by mutations in this gene.


Images

GRHPR antibody Western blotting analysis using anti-GRHPR antibody (Cat#63508). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GRHPR antibody (Cat#63508, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
63508 WB KD 1 Western blotting analysis using anti-GRHPR antibody (Cat#63508). GRHPR expression in wild type (WT) and GRHPR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GRHPR antibody (Cat#63508, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using anti-FeQ™ ECL Substrate Kit (Cat#226).


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