Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG2a |
| Clonality: | Monoclonal antibody |
| Gene Name: | far upstream element binding protein 3 |
| Gene Symbol: | FUBP3 |
| Synonyms: | FBP3 |
| Gene ID: | 8939 |
| UniProt ID: | Q96I24 |
| Clone ID: | 24GB13150 |
| Immunogen: | Recombinant protein of human FUBP3 |
| Dilution: | WB 1:200-1:1,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables single-stranded DNA binding activity. Involved in DNA-templated transcription; positive regulation of DNA-templated transcription; and positive regulation of gene expression. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus.
Images
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Western blotting analysis using anti-FUBP3 antibody (Cat#64829). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FUBP3 antibody (Cat#64829, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-FUBP3 antibody (Cat#64829). FUBP3 expression in wild-type (WT) and FUBP3 shRNA knockdown (KD) HeLa cells with 10 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FUBP3 antibody (Cat#64829, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of FUBP3 expression in HT-1080 cells using anti-FUBP3 antibody (Cat#64829, 1:2,000). Green, isotype control; red, FUBP3. |
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Immunocytochemical staining of HT-1080 cells with anti-FUBP3 associated protein antibody (Cat#64829, 1:1,000). Nuclei were stained blue with DAPI; FUBP3 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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