Background

This gene FLI1 encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-FLI1 antibody (Cat#61569). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FLI1 antibody (Cat#61569, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-FLI1 antibody (Cat#61569). FLI1 expression in wild-type (WT) and FLI1 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FLI1 antibody (Cat#61569, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of FLI1 expression in HT-1080 cells using anti-FLI1 antibody (Cat#61569, 1:2,000). Green, isotype control; red, FLI1.
	Immunocytochemical staining of HT-1080 cells with anti-FLI1 antibody (Cat#61569, 1:1,000). Nuclei were stained blue with DAPI; FLI1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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