Background

The protein encoded by the gene FGF2 is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Images

	Western blotting analysis using anti-FGF2 antibody (Cat#63186). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FGF2 antibody (Cat#63186, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). FGF2, fibroblast growth factor 2.
	Western blotting analysis using anti-fibroblast growth factor 2 antibody (Cat#63186). Fibroblast growth factor 2 expression in wild-type (WT) and fibroblast growth factor 2 (FGF2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-fibroblast growth factor 2 antibody (Cat#63186, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of FGF2 expression in HepG2 cells using anti-FGF2 antibody (Cat#63186, 1:2,000). Green, isotype control; red, FGF2.

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