Background

This gene encodes a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46. Alternative splicing of this gene results in multiple transcript variants.

Images

	Western blotting analysis using anti-exosome component 1 antibody (Cat#63872). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-exosome component 1 antibody (Cat#63872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-exosome component 1 antibody (Cat#63872). Exosome component 1 expression in wild-type (WT) and exosome component 1 (EXOSC1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-exosome component 1 antibody (Cat#63872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Exosome component 1 expression in HepG2 cells using anti-Exosome component 1 antibody (Cat#63872, 1:1,000). Green, isotype control; red, Exosome component 1.

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