Background

This gene ERBB2 encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Images

	Western blotting analysis using anti-erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
	Western blotting analysis using anti-erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444). Erb-b2 receptor tyrosine kinase 2 expression in wild type (WT) and erb-b2 receptor tyrosine kinase 2 (ERBB2) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Erb-b2 receptor tyrosine kinase 2 expression in H9c2 cells using anti-Erb-b2 receptor tyrosine kinase 2 antibody (Cat#63444, 1:2,000). Green, isotype control; red, Erb-b2 receptor tyrosine kinase 2.

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