Background

This gene EIF4EBP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Images

	Western blotting analysis using anti-EIF4EBP1 antibody (Cat#63184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF4EBP1 antibody (Cat#63184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). EIF4EBP1, eukaryotic translation initiation factor 4E binding protein 1.
	Western blotting analysis using anti-EIF4EBP1 antibody (Cat#63184). EIF4EBP1 expression in wild-type (WT) and EIF4EBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-EIF4EBP1 antibody (Cat#63184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of EIF4EBP1 expression in C2C12 cells using anti-EIF4EBP1 antibody (Cat#63184, 1:2,000). Green, isotype control; red, EIF4EBP1.
	Immunocytochemical staining of C2C12 cells with anti-EIF4EBP1 antibody (Cat#63184, 1:1,000). Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.

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