Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5′ end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5′-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-eukaryotic translation initiation factor 4E antibody (Cat#61648). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-eukaryotic translation initiation factor 4E antibody (Cat#61648, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-eukaryotic translation initiation factor 4E antibody (Cat#61648). Eukaryotic translation initiation factor 4E expression in wild type (WT) and eukaryotic translation initiation factor 4E (EIF4E) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-eukaryotic translation initiation factor 4E antibody (Cat#61648, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Eukaryotic translation initiation factor 4E expression in HepG2 cells using anti-Eukaryotic translation initiation factor 4E antibody (Cat#61648, 1:2,000). Green, isotype control; red, Eukaryotic translation initiation factor 4E.
	Immunocytochemical staining of HepG2 cells with anti-Eukaryotic translation initiation factor 4E antibody (Cat#61648, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

You may also be interested in: