Background

The protein DGKA encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene.

Images

	Western blotting analysis using anti-diacylglycerol kinase alpha antibody (Cat#64923). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-diacylglycerol kinase alpha antibody (Cat#64923, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-diacylglycerol kinase alpha antibody (Cat#64923). Diacylglycerol kinase alpha expression in wild-type (WT) and diacylglycerol kinase alpha (DGKA) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-diacylglycerol kinase alpha antibody (Cat#64923, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Diacylglycerol kinase alpha expression in HepG2 cells using anti-Diacylglycerol kinase alpha antibody (Cat#64923, 1:2,000). Green, isotype control; red, Diacylglycerol kinase alpha.

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