Background

In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene.

Images

	Western blotting analysis using anti-DAZ associated protein 1 antibody (Cat#63228). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DAZ associated protein 1 antibody (Cat#63228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-DAZ associated protein 1 antibody (Cat#63228). DAZ associated protein 1 expression in wild-type (WT) and DAZ associated protein 1 (DAZAP1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DAZ associated protein 1 antibody (Cat#63228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of DAZ associated protein 1 expression in HepG2 cells using anti-DAZ associated protein 1 antibody (Cat#63228, 1:2,000). Green, isotype control; red, DAZ associated protein 1.
	Immunocytochemical staining of HepG2 cells with anti-DAZ associated protein 1 antibody (Cat#63228, 1:1,000). Nuclei were stained blue with DAPI; DAZ associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: . Scale bar: 20 μm.

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