Background

This gene CRABP2 encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[.

Images

	Western blotting analysis using anti-CRABP2 antibody (Cat#63267). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CRABP2 antibody (Cat#63267, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CRABP2, cellular retinoic acid binding protein 2.
	Western blotting analysis using anti-CRABP2 antibody (Cat#63267). CRABP2 expression in wild-type (WT) and CRABP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CRABP2 antibody (Cat#63267, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of CRABP2 expression in C2C12 cells using anti-CRABP2 antibody (Cat#63267, 1:2,000). Green, isotype control; red, CRABP2.

You may also be interested in: