Background

The protein encoded by this gene is one subunit of a cleavage factor required for 3′ RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3′ end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.

Images

	Western blotting analysis using anti-CPSF6 antibody (Cat#65204). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CPSF6 antibody (Cat#65204, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-CPSF6 antibody (Cat#65204). CPSF6 expression in wild-type (WT) and CPSF6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CPSF6 antibody (Cat#65204, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of CPSF6 expression in HepG2 cells using anti-CPSF6 antibody (Cat#65204, 1:2,000). Green, isotype control; red, CPSF6.
	Immunocytochemical staining of HepG2 cells with anti-CPSF6 antibody (Cat#65204, 1:1,000). Nuclei were stained blue with DAPI; CPSF6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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