Description
| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | cytidine/uridine monophosphate kinase 1 |
| Gene Symbol: | CMPK1 |
| Synonyms: | CK; CMK; UMK; CMPK; UMPK; UMP-CMPK |
| Gene ID: | 51727 |
| UniProt ID: | P30085 |
| Clone ID: | 24GB7965 |
| Immunogen: | Recombinant protein of human CMPK1 |
| Dilution: | WB 1:500-1:2,500; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants.
Images
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Western blotting analysis using anti-CMPK1 antibody (Cat#63806). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CMPK1 antibody (Cat#63806, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-CMPK1 antibody (Cat#63806). CMPK1 expression in wild type (WT) and CMPK1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CMPK1 antibody (Cat#63806, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of CMPK1 expression in HepG2 cells using anti-CMPK1 antibody (Cat#63806, 1:1,000). Green, isotype control; red, CMPK1. |
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