Background

The protein CKB encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

Images

	Western blotting analysis using anti-creatine kinase B antibody (Cat#63706). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-creatine kinase B antibody (Cat#63706, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-creatine kinase B antibody (Cat#63706). Creatine kinase B expression in wild-type (WT) and creatine kinase B (CKB) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-creatine kinase B antibody (Cat#63706, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Creatine kinase B expression in HAP-1 cells using anti-Creatine kinase B antibody (Cat#63706, 1:2,000). Green, isotype control; red, Creatine kinase B.
	Immunocytochemical staining of HAP1 cells with anti-Creatine kinase B antibody (Cat#63706, 1:1,000). Nuclei were stained blue with DAPI;Creatine kinase B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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