Background

This gene CDK5 encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-cyclin dependent kinase 5 antibody (Cat#61114). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin dependent kinase 5 antibody (Cat#61114, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-cyclin dependent kinase 5 antibody (Cat#61114). Cyclin dependent kinase 5 expression in wild type (WT) and cyclin dependent kinase 5 (CDK5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cyclin dependent kinase 5 antibody (Cat#61114, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of cyclin dependent kinase 5 expression in HepG2 cells using cyclin dependent kinase 5 antibody (Cat#61114, 1:2,000). Green, isotype control; red, cyclin dependent kinase 5.
	Immunocytochemical staining of HepG2 cells with cyclin dependent kinase 5 antibody (Cat#61114, 1:1,000). Nuclei were stained blue with DAPI; Cyclin dependent kinase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.