Background

This gene CDK2 encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-cyclin dependent kinase 2 antibody (Cat#63162). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-cyclin dependent kinase 2 antibody (Cat#63162). Cyclin dependent kinase 2 expression in wild type (WT) and cyclin dependent kinase 2 (CDK2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of cyclin dependent kinase 2 expression in HepG2 cells using anti-cyclin dependent kinase 2 antibody (Cat#63162, 1:2,000). Green, isotype control; red, cyclin dependent kinase 2.

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