Description
| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | cell division cycle associated 8 |
| Gene Symbol: | CDCA8 |
| Synonyms: | BOR; DasraB; MESRGP; BOREALIN |
| Gene ID: | 55143 |
| UniProt ID: | Q53HL2 |
| Clone ID: | 24GB6600 |
| Immunogen: | Recombinant protein of human CDCA8 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16.
Images
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Western blotting analysis using anti-cell division cycle associated 8 antibody (Cat#63576). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cell division cycle associated 8 antibody (Cat#63576, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-cell division cycle associated 8 antibody (Cat#63576). cell division cycle associated 8 expression in wild type (WT) and cell division cycle associated 8 (CDCA8) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cell division cycle associated 8 antibody (Cat#63576, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of Cell division cycle associated 8 expression in HAP-1 cells using anti-Cell division cycle associated 8 antibody (Cat#63576, 1:2,000). Green, isotype control; red, Cell division cycle associated 8. |
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Immunocytochemical staining of HAP-1 cells with Cell division cycle associated 8 antibody (Cat#63576, 1:1,000). Nuclei were stained blue with DAPI; Cell division cycle associated 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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