Description
| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG2b |
| Clonality: | Monoclonal antibody |
| Gene Name: | cell division cycle 123 |
| Gene Symbol: | CDC123 |
| Synonyms: | D123; C10orf7 |
| Gene ID: | 8872 |
| UniProt ID: | O75794 |
| Clone ID: | 24GB5705 |
| Immunogen: | Recombinant protein of human CDC123 |
| Dilution: | WB 1:2,500-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables ATP binding activity and magnesium ion binding activity. Involved in eukaryotic translation initiation factor 2 complex assembly. Located in cytoplasm.
Images
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Western blotting analysis using anti-cell division cycle 123 antibody (Cat#63454). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cell division cycle 123 antibody (Cat#63454, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-cell division cycle 123 antibody (Cat#63454). Cell division cycle 123 expression in wild type (WT) and cell division cycle 123 (CDC123) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cell division cycle 123 antibody (Cat#63454, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of cell division cycle 123 expression in HepG2 cells using anti-cell division cycle 123 antibody (Cat#63454, 1:2,000). Green, isotype control; red, cell division cycle 123. |
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