Background

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5′ sequence and encoding distinct isoforms have been found for this gene.

Images

	Western blotting analysis using anti-Acetyl-CoA carboxylase alpha antibody (Cat#62029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Acetyl-CoA carboxylase alpha antibody (Cat#62029, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Acetyl-CoA carboxylase α antibody (Cat#62029). Acetyl-CoA carboxylase α expression in wild type (WT) and Acetyl-CoA carboxylase α shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Acetyl-CoA carboxylase α antibody (Cat#62029, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Acetyl-CoA carboxylase alpha expression in HepG2 cells using Acetyl-CoA carboxylase alpha antibody (Cat#62029, 1:2,000). Green, isotype control; red, Acetyl-CoA carboxylase alpha.
	Immunocytochemical staining of HepG2 cells with anti-Acetyl-CoA carboxylase alpha antibody (Cat#62029, 1:1,000). Nuclei were stained blue with DAPI; Acetyl-CoA carboxylase alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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