Background

The membrane-associated protein encoded by the gene ABCB1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and

Images

	Western blotting analysis using anti-ABCB1 antibody. Total cell lysates (30 ug) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ABCB1 antibody (1:5,000) and HRP-conjugated goat antirabbit secondary antibody (1:20,000) respectively.
	Western blotting analysis using anti-ABCB1 antibody.ABCB1expression in wild type (WT) and ABCB1 shRNA knockdown (KD) Hela cells with 30ug of total cell lysates. Hsp90 a serves as a loading control. The blot was incubated with anti-ABCB1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively.
	lmmunocytochemical staining of HepG2 cells with ABCB1 antibody (1:1,000). Nuclei were stained blue with DAPl; ABCB1 was stained magenta with Alexa Fluor647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 um.