Description

This product is a special reagent for rapid and sensitive Real-Time PCR quantitative detection of DNA using sequence-specific probes. The optimized premixed solution can shorten the reaction time by more than 60% and is suitable for standard or fast PCR instruments. FastFire qPCR PreMix uses antibody modified Anti Taq DNA polymerase, and cooperates with a unique fast PCR buffer system to ensure sensitive qPCR reaction on all Real-Time PCR instruments. It has the characteristics of fast reaction, which shortens PCR reaction time by 60%, high amplification efficiency, high amplification specificity and wide credible range, enabling faster collection of high accuracy results.

Key Features

• Fastest: Probe method FastFire series adopt antibody modified Anti Taq DNA polymerase that can be activated quickly, and cooperate with unique fast Buffer system, which can save up to 60% of reaction time and become the fastest probe reagent on the market at present.

• Strong amplification capability: Strong amplification fluorescence signal, more accurate and credible results.

• Good stability: A unique PCR stabilizer and enhancer are added to buffer to make the result more stable, repeatable, accurate and credible.

• Widely applicable: It is not only suitable for fast real time PCR instrument, but also suitable for common real time PCR instrument.

• ROX correction: ROX dye is packaged separately, which is more flexible to use and have more accurate results.

Applications

For fast, quantitative, specific real-time PCR using sequence-specific probe

Components

1. 2× FastFire qPCR PreMix (Probe): 4×1.25 ml
2. 50× ROX Reference Dye: 1 ml
3. RNase-Free ddH2O: 5×1 ml

Storage

Store -20°C

Case Study

Human GAPDH mRNA is detected by Real-Time RT-PCR. Using FastFire qPCR PreMix, the amount of cDNA is equivalent to Total RNA 1 pg-10 ng. Negative Control uses ddH2O as a template.