Description

2× LiTaq™ PCR Master Mix is an optimized ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl₂, KCI and stabilizers. 2× Taq Master Mix are stable for 20 freeze-thaw cycles when stored at -20°C. The presence of two tracking dyes allows direct loading of the PCR product onto agarose gels. The obtained PCR products are compatible with LiClone™ Ultra One Step Cloning Kit (M0011), and can be directly used for cloning into T-Vectors as most PCR products amplified with Taq DNA polymerase have one A at the 3′-terminus.

 

Key Features

• PCR products can be directly used for cloning into T-Vectors as most PCR products amplified with Taq DNA polymerase have one A at the 3′-terminus.

• Stable for 20 freeze-thaw cycles when stored at -20°C.

• Very low cost and ideal for genotyping.

 

Applications

Routine PCR

 

Component

1. 2x LiTaq™ PCR Master Mix (-Dye): 50×1 ml

 

Storage

-20°C.

 

Other Sizes

Product Name	Price	View
2× LiTaq™ PCR Master Mix (+Dye) (5 ml)	$99
2× LiTaq™ PCR Master Mix (+Dye) (15 ml)	$229
2× LiTaq™ PCR Master Mix (+Dye) (50ml)	$539
2× LiTaq™ PCR Master Mix (-Dye) (15 ml)	$229

 

Case Study

Samples: Human genomic DNA & cDNA, Mouse genomic DNA, Wheat genomic DNA, Rice genomic DNA, Coli colony

Genzera: LiTaq™ PCR mix, LiTaq™ DNA polymerase, 2× LiTaq™ plus PCR mix, 2× LiTaq™ Eco PCR mix

Competitors: Brand C, Brand N, Brand Q, Brand T, Brand G, Brand F

PCR reaction setup for amplification: • 2× Mix: 12.5 µl • F1: 1 µl • R1 : 1 µl • Template* : 0.5 µl • ddH2O: 0.2 µl * gDNA: 50 ng; λ DNA: 5 ng; Plasmid: 2 ng;  Colony: 1 µl

PCR cycling for amplification: 1. 95°C: 3 min 2. 35 x Denaturation: 15 s at 95°C Annealing: 20 s at 60°C Extension: 1 min/kb at 72°C 3. Final extension: 5 min at 72°C

 

From the above results, it can be concluded that for PCR amplification of different length and kind of templates, all four PCR mixes from our company performed superbly. For general PCR, our products have the broadest applicability, best success rate, highest specificity and maximal yield. In the most difficult situations, when all other PCR mixes failed, at least one of our products still worked.